Peptide Secretion Analysis of Cultured Cells using SensSerum® and RP-SensBeads®

SensScreen Technologies developed a patented platform for the analysis of secreted peptides from cell culture supernatants using SensSerum®
and RP-SensBeads®:

• No background compared to Fetal Calf Serum (FCS) with SensSerum®
• Rapid desalting or buffer exchange of bound peptides with RP-SensBeads®
• Very sensitive detection of secreted peptides (as low as 10 pM)
  using mass spectrometric analysis

Example for tumor cells:

Tumor cells are changing their peptide and protein secretion pattern as they progress to states of increasing malignancy and also alter their secretion rate of low molecular peptides (hormones).

To avoid high abundant peptides from normal fetal calf serum, SensSerum® was developed, which does not alter the growth rate of tumor cells.
After cell culture, low molecular substances in the supernatant can be isolated with RP-SensBeads® and analyzed by mass spectrometry.

Using the novel SensSerum®, RP-SensBeads® and subsequent mass spectremetric analysis results in a detection of peptides as low as 10 pM.

Example of non-invasive and invasive mamma carcinoma cell lines:

The non-invasive and invasive mamma carcinoma cell line shows distinctive differences in the peptide secretion pattern.
Several signals are received only for the non-invasive or the invasive cancer cell line.