Rapid Visualization of Secreted Proteins from Cell Cultures using RP-SensBeads®

Advances for the visualization of proteins from cell culture supernatants when using RP-SensBeads®:

• Efficient isolation of low concentrated proteins from cell culture media
• Rapid desalting or buffer exchange of bound proteins
• Sensitive and rapid visualization of secreted proteins possible
• Replacing time consuming ultrafiltration

In mammalian cell culture the detection of secretory proteins is normally performed by SDS gel electrophoresis.

Before gel electrophoresis, the cell culture supernatant must be desalted and concentrated.

Using the novel and patented  RP-SensBeads® from SensScreen Technologies GmbH avoids time consuming ultrafiltration.

The novel texture of the RP-SensBeads® allows, as compared to other magnetic hydrophobic particles or concentration methods, the rapid and ultrasensitive isolation of secreted proteins and peptides.

Comparison of different purification methods, using ultrafiltration devices or different magnetic reversed-phase particles.

Ultrafiltration:

Magnetic reversed-phase particles:

Rapid visualization of secreted proteins after incubation of different progressed carcinoma cell lines using different reversed-phase particles: